Product Name :
anti-ubiquityl-histone h2a (lys119) rabbit mab
Isotype :
IgG
Conjugate :
Unconjugated
Synonyms:
H2AK119ub
UniProt ID :
P04908
Immunogen:
Ubiquitinated human histone H2A (Lys119) peptide
MW (kDa) :
23
Specificity:
Anti-Ubiquityl-Histone H2A (Lys119) Rabbit mAb detects histone H2A only when it is ubiquitinated at Lys119.
Purity :
Protein A purified
Purity :
PBS, Glycerol, BSA
Storage :
Store at -20°C. Avoid freeze/thaw cycles.
Stability:
Stable for 12 months from date of receipt/reconstitution.
Background :
Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Ubiquitination, a post-translational modification in which the ubiquitin is covalently attached to a substrate protein, occurs on histones H2A and H2B. Histone H2A is mono-ubiquitinated at Lys119 by the Polycomb Repressor Complex 1 (PRC1) and is critical for gene silencing, X chromosome inactivation, and cellular response to DNA damage. Histone H2A is also monoubiquitinated at Lys119 at sites of DNA damage. Cellular location Nucleus
Images :
WB Lysate: HeLa, N2a, BRL, mouse liver, mouse kidney Protein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 8 seconds Predicted band size: 23 kDa Observed band size: 23 kDa IHC-P Tissue: Human hepatocellular carcinoma Section type: Formalin fixed & paraffin-embedded sectionRetrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (blue) Description: The brown color represents the positive signal observed with
Vapor Pressure :
Recombinant Rabbit Monoclonal Anti-Ubiquityl-Histone H2A (Lys119) Rabbit mAb Clone Number: JRMR-353 Host: Rabbit Clonality: Recombinant Monoclonal Applications: WB IHC-P ICC/IF IP ChIP Reactivity: Human, Mouse, Rat Synonyms: H2AK119ub Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H2AK119ub UniProt ID P04908 Immunogen Ubiquitinated human histone H2A (Lys119) peptide MW (kDa) 23 Specificity Anti-Ubiquityl-Histone H2A (Lys119) Rabbit mAb detects histone H2A only when it is ubiquitinated at Lys119. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat IHC-P 1:50 – 1:100 Human, Mouse ICC/IF 1:100 Human ChIP 6 ug/5X106 cells Human IP 1:50 – 1:100 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Ubiquitination, a post-translational modification in which the ubiquitin is covalently attached to a substrate protein, occurs on histones H2A and H2B. Histone H2A is mono-ubiquitinated at Lys119 by the Polycomb Repressor Complex 1 (PRC1) and is critical for gene silencing, X chromosome inactivation, and cellular response to DNA damage. Histone H2A is also monoubiquitinated at Lys119 at sites of DNA damage. Cellular location Nucleus Images WB Lysate: HeLa, N2a, BRL, mouse liver, mouse kidney Protein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 8 seconds Predicted band size: 23 kDa Observed band size: 23 kDa IHC-P Tissue: Human hepatocellular carcinoma Section type: Formalin fixed & paraffin-embedded sectionRetrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (blue) Description: The brown color represents the positive signal observed with
Recombinant Rabbit Monoclonal Anti-Ubiquityl-Histone H2A (Lys119) Rabbit mAb Clone Number: JRMR-353 Host: Rabbit Clonality: Recombinant Monoclonal Applications: WB IHC-P ICC/IF IP ChIP Reactivity: Human, Mouse, Rat Synonyms: H2AK119ub Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H2AK119ub UniProt ID P04908 Immunogen Ubiquitinated human histone H2A (Lys119) peptide MW (kDa) 23 Specificity Anti-Ubiquityl-Histone H2A (Lys119) Rabbit mAb detects histone H2A only when it is ubiquitinated at Lys119. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat IHC-P 1:50 – 1:100 Human, Mouse ICC/IF 1:100 Human ChIP 6 ug/5X106 cells Human IP 1:50 – 1:100 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Ubiquitination, a post-translational modification in which the ubiquitin is covalently attached to a substrate protein, occurs on histones H2A and H2B. Histone H2A is mono-ubiquitinated at Lys119 by the Polycomb Repressor Complex 1 (PRC1) and is critical for gene silencing, X chromosome inactivation, and cellular response to DNA damage. Histone H2A is also monoubiquitinated at Lys119 at sites of DNA damage. Cellular location Nucleus Images WB Lysate: HeLa, N2a, BRL, mouse liver, mouse kidney Protein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 8 seconds Predicted band size: 23 kDa Observed band size: 23 kDa IHC-P Tissue: Human hepatocellular carcinoma Section type: Formalin fixed & paraffin-embedded sectionRetrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (blue) Description: The brown color represents the positive signal observed with
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