Product Name :
anti-atg4b rabbit mab
Isotype :
IgG/kappa
Conjugate :
Unconjugated
Synonyms:
APG4B, AUTL1, KIAA0943
UniProt ID :
Q9Y4P1
Immunogen:
MW (kDa) :
Specificity:
Purity :
Protein G purified
Purity :
PBS, Glycerol, BSA
Storage :
Store at -20°C. Avoid freeze/thaw cycles.
Stability:
Stable for 12 months from date of receipt/reconstitution.
Background :
Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3, GABARAPL1, GABARAPL2 and GABARAP, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms. Cellular location Cytoplasm
Images :
WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 4°C overnightSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: HepG2, JurkatProtein loading quantity: 20 μgExposure time: 60 sPredicted band size: 48 kDaObserved band size: 48 kDa ICC/IF Cell line: HepG2Fixative: 100% Ice-cold methanolPermeabilization: 0.1% TritonX-100Primary Ab dilution: 1:50Primary incubation condition: 4°C overnightSecondary Ab: Goat Anti-Mouse IgGNuclear counter stain: DAPI (Blue)Description: The green color represents the positive signal observed with
Vapor Pressure :
Recombinant Rabbit Monoclonal Anti-ATG4B Rabbit mAb Clone Number: 11858 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB ICC/IF Reactivity: Human, Mouse Synonyms: APG4B, AUTL1, KIAA0943 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG/kappa Conjugate Unconjugated Synonyms APG4B, AUTL1, KIAA0943 UniProt ID Q9Y4P1 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human ICC/IF 1:50 Human, Mouse Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3, GABARAPL1, GABARAPL2 and GABARAP, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms. Cellular location Cytoplasm Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 4°C overnightSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: HepG2, JurkatProtein loading quantity: 20 μgExposure time: 60 sPredicted band size: 48 kDaObserved band size: 48 kDa ICC/IF Cell line: HepG2Fixative: 100% Ice-cold methanolPermeabilization: 0.1% TritonX-100Primary Ab dilution: 1:50Primary incubation condition: 4°C overnightSecondary Ab: Goat Anti-Mouse IgGNuclear counter stain: DAPI (Blue)Description: The green color represents the positive signal observed with
Recombinant Rabbit Monoclonal Anti-ATG4B Rabbit mAb Clone Number: 11858 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB ICC/IF Reactivity: Human, Mouse Synonyms: APG4B, AUTL1, KIAA0943 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG/kappa Conjugate Unconjugated Synonyms APG4B, AUTL1, KIAA0943 UniProt ID Q9Y4P1 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human ICC/IF 1:50 Human, Mouse Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3, GABARAPL1, GABARAPL2 and GABARAP, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms. Cellular location Cytoplasm Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 4°C overnightSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: HepG2, JurkatProtein loading quantity: 20 μgExposure time: 60 sPredicted band size: 48 kDaObserved band size: 48 kDa ICC/IF Cell line: HepG2Fixative: 100% Ice-cold methanolPermeabilization: 0.1% TritonX-100Primary Ab dilution: 1:50Primary incubation condition: 4°C overnightSecondary Ab: Goat Anti-Mouse IgGNuclear counter stain: DAPI (Blue)Description: The green color represents the positive signal observed with
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