anti-peg10 rabbit mab

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Recombinant Rabbit Monoclonal Anti-PEG10 Rabbit mAb Clone Number: JRMR-10844-18 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB Reactivity: Human, Mouse, Rat Synonyms: EDR Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms EDR UniProt ID Q86TG7 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human, Mouse, Rat Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Paternally expressed gene 10(PEG10 ) is an imprinted gene which is derived from a Ty3/Gypsy long terminal repeat (LTR) retrotransposon family encoding Gag- and Pol-like domains. Deletion of PEG10 in mice leads to embryonic lethality due to defects in placental formation. PEG10 is aberrantly expressed in several cancer types, including hepatocellular carcinoma, and contributes to tumorigenesis by affecting cell proliferation, apoptosis, and metastasis. The PEG10 gene has two overlapping open reading frames that are regulated by the programmed process of -1 frameshifting. The first encoded protein ORF1 (or RF1) has a Gag domain with coiled-coil domain and zinc finger domains, while ORF1-2 (or RF1/RF2) is produced by -1 frameshifting and creates a fusion of the ORF1 Gag domain with a carboxyl-terminal Pol-like protease domain. PEG10 has retained the ability to form virus-like particles (VLPs) that are secreted as small extracellular vesicles delivered to distant sites. Cellular location Cytoplasm, Cell membrane, Nucleus Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgGH&L (HRP Conjugate) Lysate: 1: HepG2, 2: HeLa, 3: Mouse placenta, 4: Rat placenta Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 55/100 kDa Observed band size: 55/100 kDa :
Recombinant Rabbit Monoclonal Anti-PEG10 Rabbit mAb Clone Number: JRMR-10844-18 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB Reactivity: Human, Mouse, Rat Synonyms: EDR Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms EDR UniProt ID Q86TG7 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human, Mouse, Rat Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Paternally expressed gene 10(PEG10 ) is an imprinted gene which is derived from a Ty3/Gypsy long terminal repeat (LTR) retrotransposon family encoding Gag- and Pol-like domains. Deletion of PEG10 in mice leads to embryonic lethality due to defects in placental formation. PEG10 is aberrantly expressed in several cancer types, including hepatocellular carcinoma, and contributes to tumorigenesis by affecting cell proliferation, apoptosis, and metastasis. The PEG10 gene has two overlapping open reading frames that are regulated by the programmed process of -1 frameshifting. The first encoded protein ORF1 (or RF1) has a Gag domain with coiled-coil domain and zinc finger domains, while ORF1-2 (or RF1/RF2) is produced by -1 frameshifting and creates a fusion of the ORF1 Gag domain with a carboxyl-terminal Pol-like protease domain. PEG10 has retained the ability to form virus-like particles (VLPs) that are secreted as small extracellular vesicles delivered to distant sites. Cellular location Cytoplasm, Cell membrane, Nucleus Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgGH&L (HRP Conjugate) Lysate: 1: HepG2, 2: HeLa, 3: Mouse placenta, 4: Rat placenta Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 55/100 kDa Observed band size: 55/100 kDa