anti-phospho-akt1 (ser473) rabbit mab

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Recombinant Rabbit Monoclonal Anti-Phospho-AKT1 (Ser473) Rabbit mAb Clone Number: JRMR-10760-38-H0L0 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB Reactivity: Human, Mouse, Rat Synonyms: RAC-alpha serine/threonine-protein kinase Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms RAC-alpha serine/threonine-protein kinase UniProt ID P31749 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. AKT has an important role in the regulation of NF-kappa-B-dependent gene transcription and positively regulates the activity of CREB1 (cyclic AMP (cAMP)-response element binding protein). Cellular location Nucleus, Cell membrane, Cytoplasm Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L(HRP Conjugate) Lysate: (-): Jurkat, (+): Jurkat+ Calyculin A(100nM, 30min) Protein loading quantity: 20 μg Exposure time: 30 secondsPredicted band size: 56 kDa Observed band size: 56, 60 kDa Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L(HRP Conjugate) Lysate:(-): MCF-7, (+): MCF-7+ Calyculin A(100nM, 30min) Protein loading quantity: 20 μg Exposure time: 30 secondsPredicted band size: 56 kDa Observed band size: 56, 60 kDa Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L(HRP Conjugate) Lysate:(-): HeLa, (+): HeLa+ Calyculin A(100nM, 30min) Protein loading quantity: 20 μg Exposure time: 30 secondsPredicted band size: 56 kDa Observed band size: 56, 60 kDa Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L(HRP Conjugate) Lysate:(-): NIH/3T3, (+): NIH/3T3+ CalyculinA(100ng/ml, 60min) Protein loading quantity: 20 μg Exposure time: 30 secondsPredicted band size: 56 kDa Observed band size: 56, 60 kDa Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb(HRP Conjugate) Lysate:(-): C6, (+): C6+ Calyculin A (100nM, 30min) Protein loading quantity: 20 μg Exposure time: 30 secondsPredicted band size: 56 kDa Observed band size: 56, 60 kDa Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb(HRP Conjugate) Lysate:1: Jurkat+ Calyculin A (100nM, 30min) ,then the membrane treated with Lambda Protein Phosphatase (400 U/ml) at 30°C for 1hour; 2: Jurkat+ Calyculin A (100nM, 30min) Protein loading quantity: 20 μg Exposure time: 30 secondsPredicted band size: 56 kDa Observed band size: 56, 60 kDa :
Recombinant Rabbit Monoclonal Anti-Phospho-AKT1 (Ser473) Rabbit mAb Clone Number: JRMR-10760-38-H0L0 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB Reactivity: Human, Mouse, Rat Synonyms: RAC-alpha serine/threonine-protein kinase Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms RAC-alpha serine/threonine-protein kinase UniProt ID P31749 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. AKT has an important role in the regulation of NF-kappa-B-dependent gene transcription and positively regulates the activity of CREB1 (cyclic AMP (cAMP)-response element binding protein). Cellular location Nucleus, Cell membrane, Cytoplasm Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L(HRP Conjugate) Lysate: (-): Jurkat, (+): Jurkat+ Calyculin A(100nM, 30min) Protein loading quantity: 20 μg Exposure time: 30 secondsPredicted band size: 56 kDa Observed band size: 56, 60 kDa Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L(HRP Conjugate) Lysate:(-): MCF-7, (+): MCF-7+ Calyculin A(100nM, 30min) Protein loading quantity: 20 μg Exposure time: 30 secondsPredicted band size: 56 kDa Observed band size: 56, 60 kDa Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L(HRP Conjugate) Lysate:(-): HeLa, (+): HeLa+ Calyculin A(100nM, 30min) Protein loading quantity: 20 μg Exposure time: 30 secondsPredicted band size: 56 kDa Observed band size: 56, 60 kDa Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L(HRP Conjugate) Lysate:(-): NIH/3T3, (+): NIH/3T3+ CalyculinA(100ng/ml, 60min) Protein loading quantity: 20 μg Exposure time: 30 secondsPredicted band size: 56 kDa Observed band size: 56, 60 kDa Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb(HRP Conjugate) Lysate:(-): C6, (+): C6+ Calyculin A (100nM, 30min) Protein loading quantity: 20 μg Exposure time: 30 secondsPredicted band size: 56 kDa Observed band size: 56, 60 kDa Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb(HRP Conjugate) Lysate:1: Jurkat+ Calyculin A (100nM, 30min) ,then the membrane treated with Lambda Protein Phosphatase (400 U/ml) at 30°C for 1hour; 2: Jurkat+ Calyculin A (100nM, 30min) Protein loading quantity: 20 μg Exposure time: 30 secondsPredicted band size: 56 kDa Observed band size: 56, 60 kDa