Product Name: GSK3alpha Antibody [6G12C2]
Species Reactivity: Human
Tested Applications: ELISA, WB
Applications: Western Blot:1:500 – 1:2,000 ELISA:Propose dilution 1:10,000.Determining optimal working dilutions by titration test.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight:
Immunogen: Ni-NTA purified truncated recombinant GSK3 alpha expressed in E. Coli strain BL21 (DE3).
Host Species: Mouse
Purification:
Physical State:
CAS NO.: 272105-42-7
Y-33075 (dihydrochloride)
Buffer: Ascitic fluid containing 0.03% sodium azide.
Concentration:
Storage Conditions: GSK3 alpha monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Clonality: Monoclonal
Conjugate: Unconjugated
Alternate Names: GSK-3 alpha,
Accession NO.: P49840
Protein Ino: 12644292
Official Symbol: GSK3A
Geneid: 2931
Background: Glycogen synthase kinase 3 alpha belongs to the Ser/Thr family of protein kinases, Cdc2/cdkx subfamily，GSK3 subsubfamily. It is implicated in the hormonal control of several regulatory proteins including glycogen synthase, myb, and the transcription factor c jun. GSK3 phosphorylates glycogen synthase and thereby inactivates it. Insulin stimulates the dephosphorylation of glycogen synthase at the sites phosphorylated by GSK3 and subsequently inhibits GSK3 acutely leading to the stimulation of glycogen synthesis. GSK3 signaling is performed by two isoforms, GSK3 alpha and GSK3 beta. The two isoforms share 97% sequence similarity within their catalytic domains. GSK3 has also been shown to play a role in protein synthesis, cell adhesion, cell proliferation, cell differentiation, microtubule dynamics and cell motility.
PubMed ID:http://aac.asm.org/content/20/3/387.abstract
