Product Name: GAPDH Antibody [1D4]
Species Reactivity: Bovine, Chicken, Human, Mouse, Porcine, Rat
Tested Applications: IHC, WB
Applications:
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight: 38
Immunogen: Extensively purified pig GAPDH.
Host Species: Mouse
Purification: Total IgM fraction–concentrated culture supernatant
Physical State: Liquid
CAS NO.: 252917-06-9
product targets : Hexokinase inhibitors
Buffer:
Concentration:
Storage Conditions: GAPDH antibody can be stored at -20˚C and is stable at -20˚C for at least 1 year.
Clonality: Monoclonal
Conjugate: Unconjugated
Alternate Names: GAPD2, GAPDS, HSD-35, GAPDH-2, GAPD2, GAPDH2, GAPDS
Accession NO.: O14556
Protein Ino:
Official Symbol: GAPDHS
Geneid: 26330
Background: Biological Significance: Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) is a metabolic enzyme responsible for catalyzing one step in the glycolytic pathway, the reversible oxidative phosphorylation of glyceraldehyde 3-phosphate. Because GAPDH is a protein expressed in large amounts and which is required at all times for important house keeping functions, levels of GAPDH mRNA are often measured and used as standards in studies of mRNA expression. Increasingly, scientists are making use of specific antibodies to GAPDH in comparable studies of levels of protein expression. This antibody can be used as a loading control for western blotting experiments, allowing comparison between the level of this protein and others in a cell or tissue. Apart from a role in glycolysis, GAPDH may have other roles such as in the activation of transcription. GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimers disease, and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntingtons disease. Associations with actin and tubulin have also been reported. The protein may also have a role in the regulation of apoptosis, and interestingly migrates from the cytoplasm into the nucleus when cells become apoptotic.
PubMed ID:http://aac.asm.org/content/41/12/2776.abstract
