Product Name: GABA A Receptor alpha 1 Antibody
Species Reactivity: Bovine, Human, Rat
Tested Applications: WB
Applications: GABAA Receptor antibody is specific for the 51k band of GABAA-R-α1-subunit in rat brain membrane fractions. Initial recommended range of dilutions: 1:500 to 1:2000. Rabbit anti-GABAA Receptor α1 (CT) recognizes bovine, human, mouse and rat GABAA-R-α1-subunit. Applications include Dot Blots (DB) and Western Blot (WB). Suitability for Immunohistochemistry (IHC) not yet determined. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight: 51
Immunogen: GABAA Receptor a1 (CT) polyclonal antibody was raised against a fusion protein from the cytoplasmic loop of the rat GABAA-R α1 subunit.
Host Species: Rabbit
Purification: Affinity Purified
Physical State: Liquid
CAS NO.: 1035270-39-3
product targets : CRM1 inhibitors
Buffer: 100 uL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mL BSA and 50% glycerol.
Concentration:
Storage Conditions: For long term storage –80˚C is recommended, but shorter term storage at –20˚C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: Gabra-1,
Accession NO.: P62813
Protein Ino: 51317298
Official Symbol: Gabra1
Geneid: 29705
Background: Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl- channel associated with the GABAA-Receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date six alphas, four betas and four gammas, plus alternative splicing variants of some of these subunits, have been identified. Injection in oocytes or mammalian cell lines of cRNA coding for alpha and beta subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a gamma subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different alpha subunits of the receptor. Lastly, phosphorylation of beta subunits of the receptor has been shown to modulate GABAA-R function.
PubMed ID:http://aac.asm.org/content/41/10/2184.abstract
