Product Name: Fibrillarin Antibody [38F3]
Species Reactivity: Drosophila, Human, Rat
Tested Applications: IHC, WB
Applications:
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight: 34
Immunogen: Yeast nuclear preparations.
Host Species: Mouse
Purification: Total IgG fraction
Physical State: Liquid
CAS NO.: 956697-53-3
LDE225
Buffer:
Concentration:
Storage Conditions: Fibrillarin antibody can be stored at -20˚C and is stable at -20˚C for at least 1 year.
Clonality: Monoclonal
Conjugate: Unconjugated
Alternate Names: LOT3, LOT3
Accession NO.: P15646
Protein Ino: 119857
Official Symbol: NOP1
Geneid: 851548
Background: Nop1p was originally identified as a nucleolar protein of bakers yeast, Saccharomyces cerevisiae. The Nop1p protein is 327 amino acids in size (34.5 kDa), is essential for yeast viability, and is localized in the nucleoli. The systematic name for S. cerevisiae Nop1 is YDL014W, and it is now known to be part of the small subunit processome complex, involved in the processing of pre-18S ribosomal RNA. Nop1p is the yeast homologue of a protein found in all eukaryotes and archea generally called fibrillarin. Fibrillarin/Nop1p is extraordinarily conserved, so that the yeast and human proteins are 67% identical, and the human protein can functionally replace the yeast protein. Patients with the autoimmune disease scleroderma often have strong circulating autoantibodies to a ~34 kDa protein which was subsequently found to be fibrillarin. Recent studies show that knock-out of the fibrillarin gene in mice results in embryonic lethality, although mice with only one functional fibrillarin/Nop1p gene were viable. This antibody is becoming widely used as a convenient marker for nucleoli in a wide variety of species (e.g. 4-6).
PubMed ID:http://aac.asm.org/content/41/2/326.abstract
