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Product Name: DLK1 Antibody [PF299-1]
Species Reactivity: Human
Tested Applications: ELISA, Flow, IHC, WB
Applications: ELISA: (direct or indirect: 1:2,000-1:10,000). Flow Cytometry: (1:100-1:500). Immunohistochemistry: (paraffin sections (1:100-1:500)). Western blot: (1:2,000-1:10,000 using ECL. Suggested blocking and dilution buffer is TBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature). Optimal conditions should be determined individually for each application.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight:
Immunogen: Recombinant human DLK1 (extracellular domain).
Host Species: Mouse
Purification:
Physical State: Liquid
CAS NO.: 1254473-64-7
Product: LY2874455
Buffer: Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.
Concentration: 1 mg/ml
Storage Conditions: Stable for at least 1 year after receipt when stored at -20˚C.
Clonality: Monoclonal
Conjugate: Unconjugated
Alternate Names: Protein Delta Homolog 1; Fetal Antigen 1; Preadipocyte Factor 1; Pref-1; FA1
Accession NO.: NP_003827
Protein Ino: 74136023
Official Symbol: DLK1
Geneid: 8788
Background: DLK1 (Protein delta homolog 1; Preadipocyte factor 1; Pref-1) is a regulator of adipocyte differentiation found in serum and urine. DLK1 might be a decoy ligand. Serum or plasma levels of soluble DLK1 affect negatively or positively adipogenesis and control mesenchymal cell fate. DLK1 is frequently upregulated in myelodysplastic syndrome (MDS) patients compared to non-leukemic individuals.
PubMed ID:http://aac.asm.org/content/38/9/2024.abstract

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Author: Betaine hydrochloride