Product Name: DFF45 Antibody
Species Reactivity: Human
Tested Applications: ELISA, ICC, IF, WB
Applications: DFF45 antibody can be used for detection of DFF45 and one of the cleaved fragments of DFF45 by Western blot at at 0.5 – 1 μg/mL and for immunoprecipitation. A 45 kDa band can be detected in non-apoptotic cells. Antibody can also be used for immunocytochemistry starting at 5 μg/mL.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight: 45 kDa
Immunogen: DFF45 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human DFF45.The immunogen is located within the last 50 amino acids of DFF45.
Host Species: Rabbit
Purification: DFF45 Antibody is Antibody is DEAE purified.
Physical State: Liquid
CAS NO.: 163222-33-1
Product: Ezetimibe
Buffer: DFF45 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
Storage Conditions: DFF45 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: DFF45 Antibody: DFF1, ICAD, DFF-45, DFF1, DFF45, H13, DNA fragmentation factor subunit alpha, DNA fragmentation factor 45 kDa subunit
Accession NO.: NP_004392
Protein Ino: 4758148
Official Symbol: DFFA
Geneid: 1676
Background: DFF45 Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A human 45 kDa DNA fragmentation factor (DFF45) was identified recently which was cleaved by caspase-3 during apoptosis. Mouse homologue of human DFF45 was identified as a DNase inhibitor designated ICAD. Upon cleavage of DFF45/ICAD, a caspase activated deoxyribonuclease (DFF40/CAD) is released and activated and eventually causes the degradation of DNA in the nuclei. Therefore, the cleavage of DFF45/ICAD, which causes DFF40/CAD activation and DNA degradation, is the hallmark of apoptotic cell death.
PubMed ID:http://aac.asm.org/content/38/6/1413.abstract
