Product Name: DFF40 Antibody
Species Reactivity: Human, Mouse, Rat
Tested Applications: ELISA, ICC, IF, WB
Applications: DFF40 antibody can be used for detection of DFF40 by Western blot 0.5 μg/mL. A 40 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight: Predicted: 38 kDa Observed: 40 kDa
Immunogen: DFF40 antibody was raised against a peptide corresponding to 18 amino acids near the center of murine CAD.The immunogen is located within amino acids 130 – 180 of DFF40.
Host Species: Rabbit
Purification: DFF40 Antibody is affinity chromatography purified via peptide column.
Physical State: Liquid
CAS NO.: 172732-68-2
Product: Varespladib
Buffer: DFF40 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Conditions: DFF40 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: DFF40 Antibody: CAD, CPAN, 40kDa, DFF40, Didff, 5730477D02Rik, Cad, DNA fragmentation factor subunit beta, Caspase-activated deoxyribonuclease, CAD
Accession NO.: NP_031885
Protein Ino: 160948620
Official Symbol: Dffb
Geneid: 13368
Background: DFF40 Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A mouse DNase that causes DNA fragmentation was identified recently and designated CAD (for caspase activated deoxyribonuclease). The human homologue of mouse CAD was more recently identified by three groups independently and termed CPAN, DFF40, and human CAD, respectively. DFF45/ICAD is the inhibitory protein of DFF40/CAD and forms complex with DFF40/CAD. Upon cleavage of DFF45/ICAD by activated caspase, DFF40/CAD is released and activated and eventually causes the degradation of DNA in the nuclei. Activation of DFF40/CAD, which causes DNA degradation, is the hallmark of apoptotic cell death.
PubMed ID:http://aac.asm.org/content/38/6/1408.abstract