Product Name: DDOST Antibody
Species Reactivity: Dog, Human, Mouse, Rat, Zebrafish
Tested Applications: ELISA, WB
Applications: DDOST antibody can be used for detection of DDOST by ELISA at 1:62500. DDOST antibody can be used for detection of DDOST by western blot at 5.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 – 100,000.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight: 46 kDa
Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human DDOST.
Host Species: Rabbit
Purification: Antibody is purified by protein A chromatography method.
Physical State: Lyophilized
CAS NO.: 355406-09-6
Product: YM-155 (hydrochloride)
Buffer: Antibody is lyophilized in PBS buffer with 2% sucrose. Add 100 μL of distilled water. Final antibody concentration is 1 mg/mL.
Concentration: 1 mg/ml
Storage Conditions: For short periods of storage (days) store at 4˚C. For longer periods of storage, store DDOST antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: DDOST, AGE-R1, KIAA0115, MGC2191, OK/SW-cl.45, OST, OST48, WBP1, AGER1, CDG1R, OKSWcl45
Accession NO.: NP_005207
Protein Ino: 20070197
Official Symbol: DDOST
Geneid: 1650
Background: DDOST is a component of the oligosaccharyltransferase complex which catalyzes the transfer of high-mannose oligosaccharides to asparagine residues on nascent polypeptides in the lumen of the rough endoplasmic reticulum. The protein complex co-purifies with ribosomes. DDOST is also implicated in the processing of advanced glycation endproducts (AGEs), which form from non-enzymatic reactions between sugars and proteins or lipids and are associated with aging and hyperglycemia.
PubMed ID:http://aac.asm.org/content/38/4/865.abstract
