Product Name: Cullin 1 Antibody
Species Reactivity: Human
Tested Applications: ELISA, IHC, IP, WB
Applications: Cullin 1 antibody can be used in ELISA starting at 1:000 – 1:1000, and immunohistochemistry starting at 10 μg/mL.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight:
Immunogen: Cullin 1 antibody was raised against amino acids from the C-Terminus of Cullin 1 (Human).
Host Species: Rabbit
Purification: Delipidation and Defibrination
Physical State: Liquid
CAS NO.: 91396-88-2
Product: PluriSln 1
Buffer: 0.01% sodium azide.
Concentration:
Storage Conditions: Store Cullin 1 antibody at 4 ˚C or -20 ˚C. As with all antibodies avoid freeze/thaw cycles.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: CUL1, Cullin 1, Cullin-1, CUL-1
Accession NO.: Q13616
Protein Ino: 19863257
Official Symbol: CUL1
Geneid: 8454
Background: Cullins assemble a potentially large number of ubiquitin ligases by binding to the RING protein ROC1 to catalyse polyubiquitination, as well as binding to various specificity factors to recruit substrates. Cullin 1 is an essential component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, cul1 serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits. Cul1 may also contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. Cul1 is part of the SCF complex consisting of CUL1, RBX1, SKP1 and SKP2, where it interacts directly with SKP1, SKP2 and RBX1. Cul1 also interacts with RNF7 and is part of a complex with TIP120A/CAND1 and RBX1. The unneddylated form interacts with TIP120A/CAND1 and the interaction negatively regulates the association with SKP1 in the SCF complex.
PubMed ID:http://aac.asm.org/content/37/8/1630.abstract
