Product Name: Chk1 (phospho Ser296) Antibody
Species Reactivity: Human
Tested Applications: IHC, WB
Applications: Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
User Note:
Predicted Molecular Weight: 45 kDa
Immunogen: Chk1 (phospho Ser296) antibody was raised against a peptide sequence around phosphorylation site of Serine 296 (I-F-S (p)-N-L) derived from Human Chk1.
Host Species: Rabbit
Purification: Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Physical State: Liquid
CAS NO.: 396091-73-9
Product: Pasireotide
Buffer: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration: 1 mg/mL
Storage Conditions: Store antibody at -20˚C for up to one year.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: CHEK1, Chk1, kinase Chk1, EC 2.7.11.1
Accession NO.: NP_001107593.1
Protein Ino: 166295194
Official Symbol: CHEK1
Geneid: 1111
Background: Required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. Recognizes the substrate consensus sequence [R-X-X-S/T]. Binds to and phosphorylates CDC25A, CDC25B and CDC25C. Phosphorylation of CDC25A at Ser-178 and Thr-507 and phosphorylation of CDC25C at Ser-216 creates binding sites for 14-3-3 proteins which inhibit CDC25A and CDC25C. Phosphorylation of CDC25A at Ser-76, Ser-124, Ser-178, Ser-279 and Ser-293 promotes proteolysis of CDC25A. Inhibition of CDC25 activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. Binds to and phosphorylates RAD51 at Thr-309, which may enhance the association of RAD51 with chromatin and promote DNA repair by homologous recombination. Binds to and phosphorylates TLK1 at Ser-743, which prevents the TLK1-dependent phosphorylation of the chromatin assembly factor ASF1A. This may affect chromatin assembly during S phase or DNA repair. May also phosphorylate multiple sites within the C-terminus of TP53, which promotes activation of TP53 by acetylation and enhances suppression of cellular proliferation.
PubMed ID:http://aac.asm.org/content/36/1/132.abstract
