Product Name: Caspase-8 Antibody [1G12]
Species Reactivity: Mouse
Tested Applications: ELISA, Flow, IHC, WB
Applications: ELISA: (recombinant mouse caspase-8) . Flow Cytometry: (overexpressed in cell lines) . Immunocytochemistry: (see literature reference 2) . Western Blot: (excellent) . Optimal conditions must be determined individually for each application.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight:
Immunogen: Recombinant mouse caspase-8 (p20 subunit).
Host Species: Rat
Purification: >95% (SDS-PAGE)
Physical State: Liquid
CAS NO.: 183232-66-8
Product: AM251
Buffer: Liquid. In PBS containing 0.02% sodium azide.
Concentration: 1 mg/ml
Storage Conditions: Stable for at least 1 year after receipt when stored at -20˚C.
Clonality: Monoclonal
Conjugate: Unconjugated
Alternate Names: Apoptotic Cysteine Protease; Apoptotic Protease Mch-5; CAP4; FADD-homologous ICE/ced-3-like Protease
Accession NO.: O89110
Protein Ino: 25090576
Official Symbol: Casp8
Geneid: 12370
Background: Procaspase-8 belongs to the family of caspases. Binding of FasL to Fas leads to formation of a receptor complex at the cellular membrane, which was named DISC. The DISC consists of oligomerized receptors, the DD-containing adaptor molecule FADD, procaspase-8, procaspase-10 and c-FLIP. The DISC structure provides a platform for the oligomerization of procaspase-8 that allows two procaspase-8 homodimers to be in the close proximity leading to the initial activation of procaspase-8. At the first cleavage step, the N-terminal p43/p41 and the C-terminal p30 cleavage products are generated. Importantly, these cleavage products already possess catalytic activity. At the second cleavage step, p43/p41 and p30 are processed to p10 and p20, respectively, which leads to the generation of the active caspase-8 heterotetramer (p20/p10)2.
PubMed ID:http://aac.asm.org/content/43/5/1252.abstract
