Product Name: CaM Kinase II (phospho Thr286) Antibody
Species Reactivity: Human, Mouse, Rat, Xenopus
Tested Applications: WB
Applications: Antibody dilutions and tissue load should be based on tissue type and expected phosphorylation state. Western blots of rat brain lysates with phospho- and dephosphopeptide competition were used to establish the phospho-specificity of the antibody. Applications include Dot Blot (DB) and Western Blot (WB). Dot Blots and Western Blots performed with each lot and working dilutions internally tested under ideal conditions for DB and WB were 1:1000. Suitability for Immunohistochemistry (IHC) has not been determined. Human, mouse, rat and Xenopus have 100% amino acid sequence identity with the antigen used to raise the antibody.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight: 50/60
Immunogen: CaM Kinase II (Thr286) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acid residues surrounding the phosphorylated-Thr286 found in rat brain CaM Kinase II.
Host Species: Rabbit
Purification: Affinity Purified
Physical State: Liquid
CAS NO.: 1401223-22-0
Product: PI-1840
Buffer: 100 uL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mL BSA and 50% glycerol.
Concentration:
Storage Conditions: For long term storage –80˚C is recommended, but shorter term storage at –20˚C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: PKCCD, PK2CDD, CaM kinase II subunit alpha
Accession NO.: P11275
Protein Ino: 125285
Official Symbol: Camk2a
Geneid: 25400
Background: Calcium/calmodulin kinase II (CaM Kinase II) is multi-functional calcium and calmodulin-dependent protein kinase that mediates cellular responses to second messengers. Phosphorylation of Thr286 produces an autonomously active form of CaM Kinase II. The antibody was raised against a synthetic phosphopeptide corresponding to amino acid residues surrounding the phosphorylated-Thr286 found in rat brain CaM Kinase II.
PubMed ID:http://aac.asm.org/content/43/2/314.abstract
