Product Name: CMG1 Antibody
Species Reactivity: Human, Rat
Tested Applications: ELISA, IF, IHC-P, WB
Applications: ELISA: antibody detection limit dilution 1:16000. Western Blot: Approx 70-75kDa band observed in lysates of cell line HEK293 (calculated MW of 69.2kDa according to AAK77221.1, but 51.4kDa according to NP_079379.1). The observed molecular weight corresponds to earlier findings in literature with differen Immunohistochemistry: In paraffin embedded Human Liver shows vesicular staining in cytoplasm of hepatocytes. Recommended concentration, 5-10μg/ml. Immunofluorescence: EB06339 was successfully used at 2.5mg/ml to stain rat cortical neurons (see picture and PMID: 17166276).
User Note:
Predicted Molecular Weight:
Immunogen: CMG1 antibody was raised against a 13 amino acid synthetic peptide near the C-Terminus of CMG1.
Host Species: Goat
Purification: CMG1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Physical State: Liquid
CAS NO.: 410528-02-8
Product: Palovarotene
Buffer: CMG1 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
Concentration: 500 ug/mL
Storage Conditions: Aliquot and store at -20˚C. Minimize freezing and thawing.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: IFT74, CCDC2, CMG1, CMG-1, FLJ22621, MGC111562, intraflagellar transport 74 homolog (Chlamydomonas), capillary morphogenesis protein 1, coiled-coil domain containing 2
Accession NO.: NP_079379.1, AAK77221.1
Protein Ino: 13376669
Official Symbol: IFT74
Geneid: 80173
Background:
PubMed ID:http://aac.asm.org/content/36/8/1695.abstract
