Product Name: CELA3B Antibody [CELA3B/1757]
Species Reactivity: Human
Tested Applications: ELISA, Flow, IF, IHC-P, WB
Applications: Western blot: 0.5-1 ug/mlFlow Cytometry: 0.5-1 ug/million cells in 0.1mlIF: 1-2 ug/mlELISA: 2-4 ug/ml; order BSA free formulation for coatingIHC (FFPE): 1-2 ug/ml for 30 min at RTOptimal dilution of the CELA3B antibody should be determined by the researcher.
User Note: Optimal dilutions for each application to be determined by the researcher
Predicted Molecular Weight:
Immunogen: A recombinant partial protein (aa 82-238) was used as the immunogen for the CELA3B antibody.
Host Species: Mouse
Purification: Protein G affinity chromatography
Physical State: Liquid
CAS NO.: 76824-35-6
Product: Famotidine
Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration: 0.2 mg/mL
Storage Conditions: Aliquot and Store at -20C. Avoid freez-thaw cycles.
Clonality: Monoclonal
Conjugate: Unconjugated
Alternate Names: Chymotrypsin-like elastase family member 3B, Elastase IIIB, Elastase-3B, Protease E, CELA3B, ELA3B
Accession NO.:
Protein Ino:
Official Symbol: CELA3B
Geneid: 23436
Background: This mAb recognizes a protein of ~30kDa, identified as CELA3B (Chymotrypsin like elastase family member 3B), or Elastase 3B. Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six genes which encode the structurally similar proteins: Elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, 3B has little elastolytic activity. Like most of the human elastases, 3B is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein; it has a digestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. It may also function in the intestinal transport and metabolism of cholesterol. Elastases 3A/B have been referred to as Protease E and as Elastase 1, and excretion of this protein in fecal material is frequently used as a measure of pancreatic function in clinical assays.
PubMed ID:http://aac.asm.org/content/35/10/2161.abstract
