Product Name: CD8a Antibody [RPA-T8] (FITC)
Species Reactivity: Human
Tested Applications: Flow
Applications:
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight:
Immunogen:
Host Species: Mouse
Purification: The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.
Physical State: liquid
CAS NO.: 874101-00-5
Product: RO4987655
Buffer: Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.
Concentration: 5 uL (1 ug) / test
Storage Conditions: The product should be stored undiluted at 4˚C and should be protected from prolonged exposure to light. Do not freeze.
Clonality: Monoclonal
Conjugate: FITC
Alternate Names: CD8, MAL, p32, Leu2, CD8A
Accession NO.:
Protein Ino:
Official Symbol: CD8A
Geneid: 925
Background: The RPA-T8 monoclonal antibody specifically reacts with the human CD8a molecule, a 32 kDa cell surface receptor expressed either as a heterodimer (CD8 alpha/beta) or as a homodimer (CD8 alpha/alpha) on the majority of thymocytes, a subpopulation of mature T cells, and natural killer cells. CD8 interacts with the major histocompatibility complex class I (MHC class I) molecules on antigen-presenting cells or epithelial cells. The RPA-T8 antibody reacts with 13-48% of peripheral lymphocytes, 80% of thymocytes, and a subset of natural killer cells. RPA-T8, OKT8, and HIT8a antibodies do not compete with each other for binding to peripheral leukocytes, meaning that that they do not recognize the same epitope or block each other by steric hindrance.
PubMed ID:http://aac.asm.org/content/35/6/1048.abstract
