Product Name: CD8 Antibody [C8/1035]
Species Reactivity: Human
Tested Applications: Flow, IF, WB
Applications: Flow Cytometry: 0.5-1 ug/million cellsImmunofluorescence: 0.5-1 ug/mlThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the anti-CD8 antibody to be titered up or down for optimal performance.
User Note: Optimal dilutions for each application to be determined by the researcher
Predicted Molecular Weight:
Immunogen: Recombinant full-length human CD8a protein was used as immunogen for this anti-CD8 antibody.
Host Species: Mouse
Purification: Protein G affinity chromatography
Physical State: Liquid
CAS NO.: 770-05-8
Product: Octopamine (hydrochloride)
Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration: 0.2 mg/mL
Storage Conditions: Aliquot and Store at -20C. Avoid freez-thaw cycles.
Clonality: Monoclonal
Conjugate: Unconjugated
Alternate Names: T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2, CD8a, CD8A, MAL
Accession NO.:
Protein Ino:
Official Symbol: CD8A
Geneid: 925
Background: Cluster of Differentiation 8 is a cell surface receptor expressed either as a heterodimer with the beta chain or as a homodimer. A majority of thymocytes and a subpopulation of mature Tcells and NK cells express CD8a. It binds to MHC class 1 and through its association with protein tyrosine kinase p56lck plays a role in Tcell development and activation of mature Tcells. For mature Tcells, CD4 and 8 are mutually exclusive, so antibody to CD8 is generally used in conjunction with antibody to CD4.
PubMed ID:http://aac.asm.org/content/35/4/622.abstract
