Product Name: CD34 Antibody [ICO-115]
Species Reactivity: Human, Rat
Tested Applications: Flow, IF, WB
Applications: Flow Cytometry: 0.5-1 ug/million cellsIF: 0.5-1 ug/mlThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.
User Note: Optimal dilutions for each application to be determined by the researcher
Predicted Molecular Weight:
Immunogen: Blast cells from a chronic myeloid leukemia patient were used as the immunogen for this CD34 antibody.
Host Species: Mouse
Purification: Protein G affinity chromatography
Physical State: Liquid
CAS NO.: 198062-54-3
Product: GW311616
Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration: 0.2 mg/mL
Storage Conditions: Aliquot and Store at -20C. Avoid freez-thaw cycles.
Clonality: Monoclonal
Conjugate: Unconjugated
Alternate Names: Hematopoietic progenitor cell antigen CD34, CD34, CD34
Accession NO.:
Protein Ino:
Official Symbol: CD34
Geneid: 947
Background: This antibody recognizes a single chain, transmembrane, heavily glycosylated protein of 90-120kDa, which is identified as CD34. Its expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. CD34 expression is also found in vascular endothelium. Additionally, it appears that proliferating endothelial cells overexpress this molecule than the non-proliferating endothelial cells. Anti-CD34 labels > 85% of angiosarcoma and Kaposi’s sarcoma, but shows low specificity.
PubMed ID:http://aac.asm.org/content/34/7/1414.abstract
