Product Name: CD31 Antibody [PCM25-1]
Species Reactivity: Human
Tested Applications: Flow, IF, IHC, WB
Applications: Western blot: 1-2 ug/mlFlow Cytometry: 0.5-1ug/10e6 cellsIF: 1-2 ug/mlIHC (FFPE): 1-2 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)Titering of the CD31 antibody may be required for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
User Note: Optimal dilutions for each application to be determined by the researcher
Predicted Molecular Weight:
Immunogen: Recombinant human protein was used as the immunogen for this CD31 antibody.
Host Species: Mouse
Purification: Protein G affinity chromatography
Physical State: Liquid
CAS NO.: 3992-42-5
Product: 2-Deoxycytidine (hydrochloride)
Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration: 0.2 mg/mL
Storage Conditions: Aliquot and Store at -20C. Avoid freez-thaw cycles.
Clonality: Monoclonal
Conjugate: Unconjugated
Alternate Names: Platelet endothelial cell adhesion molecule, PECAM-1, EndoCAM, GPIIA, PECA1, CD31, PECAM1
Accession NO.:
Protein Ino:
Official Symbol: PECAM1
Geneid: 5175
Background: CD31 is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. It is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [RefSeq]
PubMed ID:http://aac.asm.org/content/34/6/1276.abstract
