Product Name: CD31 Antibody [2F7B2]
Species Reactivity: Human
Tested Applications: ELISA, IHC, WB
Applications: Western Blot:1:500 – 1:2,000 IHC(P):1:500 – 1:2,000ELISA:Propose dilution 1:10,000.Determining optimal working dilutions by titration test.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight:
Immunogen: Ni-NTA purified recombinant CD31 expressed in E. Coli strain BL21 (DE3).
Host Species: Mouse
Purification:
Physical State:
CAS NO.: 313553-47-8
Product: INH1
Buffer: Ascitic fluid containing 0.03% sodium azide.
Concentration:
Storage Conditions: CD31 monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Clonality: Monoclonal
Conjugate: Unconjugated
Alternate Names: EndoCAM, PECAM-1, CD31, PECA1, GPIIA, endoCAM, CD31/EndoCAM
Accession NO.: P16284
Protein Ino: 129747
Official Symbol: PECAM1
Geneid: 5175
Background: CD31, also known as platelet endothelial cell adhesion molecule 1 (PECAM1), is a type I integral membrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors.It is constitutively expressed on the surface of endothelial cells, and concentrated at the junction between them. The antibody reacts with the murine form of the Platelet-Endothelial Cell Adhesion Molecule. The reactivity of the antibody is restricted to the isoform of the molecule that is selectively expressed by endothelial cells.The antigen is predominantly present at the lateral borders of endothelial cells as described for human PECAM-1.It is also weakly expressed on many peripheral lymphoid cells and platelets.CD31 has been used to measure angiogenesis in association with tumor recurrence. Other studies have also indicated that CD31 and CD34 can be used as markers for myeloid progenitor cells and recognize different subsets of myeloid leukemia infiltrates (granular sarcomas).
PubMed ID:http://aac.asm.org/content/34/6/1262.abstract
