Product Name: BTK Antibody
Species Reactivity: Human, Mouse
Tested Applications: ELISA, ICC, IF, IHC-P, WB
Applications: BTK antibody can be used for the detection of BTK by Western blot at 0.5 to 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL and immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 10 μg/mL.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight: Predicted: 76 kDa Observed: 79 kDa
Immunogen: BTK antibody was raised against a synthetic peptide corresponding to 16 amino acids near the amino-terminus of human BTK.The immunogen is located within amino acids 20 – 70 of BTK.
Host Species: Rabbit
Purification: BTK Antibody is affinity chromatography purified via peptide column.
Physical State: Liquid
CAS NO.: 123-31-9
Product: Hydroquinone
Buffer: BTK Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Conditions: BTK antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: BTK Antibody: AT, ATK, BPK, XLA, IMD1, AGMX1, PSCTK1, Tyrosine-protein kinase BTK, Agammaglobulinemia tyrosine kinase
Accession NO.: Q06187
Protein Ino: 547759
Official Symbol: BTK
Geneid: 695
Background: BTK Antibody: Brutons tyrosine kinase (BTK) was initially identified as a member of the src family for protein-tyrosine kinases that was involved in X-linked agamma-globulinaemia, and has since been shown to involved in a number of signaling pathways in hemapoietic lineage. It has recently been shown to interact with members of the toll-like receptor (TLR) family such as TLR4, 6, 8, and 9. The TLRs are critical molecules in both the innate and adaptive immunity and can recognize diverse microbial pathogens. BTK has also been shown to interact with key proteins involved in TLR4 signal transduction such as MyD88, TIRAP, and IRAK, but not TRAF-6, suggesting that BTK is involved in lipopolysaccharide signal transduction.
PubMed ID:http://aac.asm.org/content/42/6/1509.abstract
