Product Name: ATG16 Antibody
Species Reactivity: Human
Tested Applications: ELISA, ICC, IF, WB
Applications: ATG16 antibody can be used for the detection of ATG16 by Western blot at 1 and 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 4 μg/mL.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight:
Immunogen: ATG16 antibody was raised against a 18 amino acid synthetic peptide from near the amino terminus of human ATG16.The immunogen is located within amino acids 40 – 90 of ATG16.
Host Species: Rabbit
Purification: ATG16 Antibody is affinity chromatography purified via peptide column.
Physical State: Liquid
CAS NO.: 110044-82-1
Product: MG-101
Buffer: ATG16 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Conditions: ATG16 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: ATG16 Antibody: IBD10, WDR30, APG16L, ATG16A, ATG16L, UNQ9393/PRO34307, Autophagy-related protein 16-1, APG16-like 1
Accession NO.: NP_110430
Protein Ino: 124256480
Official Symbol: ATG16L1
Geneid: 55054
Background: ATG16 Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG16, another member of the autophagy protein family, forms a complex with the ATG5-ATG12 conjugate. This multimeric protein has been shown to be essential for autophagosome formation in both yeast and mammals and targets the ATG5-ATG12 complex to the autophagic isolation membrane during the formation of the autophagosome. Because mammalian ATG16 has seven WD-repeats in its C-terminal domain, it has been suggested that these may form a platform for further protein-protein interactions. Multiple isoforms of ATG16 are known to exist.
PubMed ID:http://aac.asm.org/content/53/7/2799.abstract
