Product Name: Eotaxin-3 Antibody [1.1_3H10-1D11]
Species Reactivity: Human
Tested Applications: ELISA, Neut, WB
Applications: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hEotaxin-3 (2.0 ng/mL), a concentration of 1.0-5.0 μg/mL of this antibody is required. ELISA:In a sandwich ELISA (assuming 100 μL/well), a concentration of 1.0-2.0 μg/mL of this antibody will detect at least 1000 pg/mL of recombinant human Eotaxin-3 when used with our biotinylated antigen affinity purified anti-human Eotaxin-3 as the detection antibody at a concentration of approximately 0.25-0.50 μg/mL. Western Blot:To detect hEotaxin-3 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEotaxin-3 is 0.25-0.5 ng/lane, under reducing or non-reducing conditions.
User Note: Centrifuge vial prior to opening.
Predicted Molecular Weight:
Immunogen: Eotaxin-3 antibody is produced in BALB/c ICRR F1 mice using highly pure (>98%) recombinant human Eotaxin-3 as the immunizing antigen.
Host Species: Mouse
Purification: This IgG2aK antibody was purified from ascites fluid by Protein A affinity chromatography.
Physical State: Lyophilized
CAS NO.: 391210-10-9
Product: PD0325901
Buffer:
Concentration:
Storage Conditions: Eotaxin-3 lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20˚C. Reconstitute in sterile water to a concentration of 0.1 – 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4˚C when reconstituted.
Clonality: Monoclonal
Conjugate: Unconjugated
Alternate Names: SCYA26, UNQ216/PRO242, C-C motif chemokine 26, CC chemokine IMAC, MIP-4-alpha, IMAC, TSC-1, MIP-4a, MIP-4alpha
Accession NO.: Q9Y258
Protein Ino: 6685990
Official Symbol: CCL26
Geneid: 10344
Background:
PubMed ID:http://aac.asm.org/content/39/11/2564.abstract
