Product Name: EIF2S2 Antibody
Species Reactivity: Human
Tested Applications: ELISA, WB
Applications: EIF2S2 antibody can be used for detection of EIF2S2 by ELISA at 1:62500. EIF2S2 antibody can be used for detection of EIF2S2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 – 100,000.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight: 38 kDa
Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human EIF2S2.
Host Species: Rabbit
Purification: Antibody is purified by peptide affinity chromatography method.
Physical State: Lyophilized
CAS NO.: 1108147-88-1
Product: MCOPPB (triHydrochloride)
Buffer: Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.
Concentration: 1 mg/ml
Storage Conditions: For short periods of storage (days) store at 4˚C. For longer periods of storage, store EIF2S2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: EIF2S2, DKFZp686L18198, EIF2, EIF2B, EIF2beta, MGC8508, PPP1R67, eIF-2-beta
Accession NO.: NP_003899
Protein Ino: 29826335
Official Symbol: EIF2S2
Geneid: 8894
Background: Eukaryotic translation initiation factor 2 (EIF-2) functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA and binding to a 40S ribosomal subunit. EIF-2 is composed of three subunits, alpha, beta, and gamma, with the protein encoded by this gene representing the beta subunit. The beta subunit catalyzes the exchange of GDP for GTP, which recycles the EIF-2 complex for another round of initiation.Western blots using two different antibodies against two unique regions of this protein target confirm the same apparent molecular weight in our tests.Eukaryotic translation initiation factor 2 (EIF-2) functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA and binding to a 40S ribosomal subunit. EIF-2 is composed of three subunits, alpha, beta, and gamma, with the protein encoded by this gene representing the beta subunit. The beta subunit catalyzes the exchange of GDP for GTP, which recycles the EIF-2 complex for another round of initiation. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
PubMed ID:http://aac.asm.org/content/39/7/1616.abstract
