Product Name: eIF2a (phospho Ser51) Antibody
Species Reactivity: Human, Mouse, Rat
Tested Applications: IF, IHC, WB
Applications: Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
User Note:
Predicted Molecular Weight: 38 kDa
Immunogen: eIF2a (Phospho-Ser51) antibody was raised against a peptide sequence around phosphorylation site of serine 51 (E-L-S (p) -R-R) derived from Human eIF2α.
Host Species: Rabbit
Purification: Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Physical State: Liquid
CAS NO.: 133407-82-6
Product: MG-132
Buffer: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration: 1 mg/mL
Storage Conditions: Store antibody at -20˚C for up to one year.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: EIF2, EIF-2, EIF2A, EIF-2A, EIF-2alpha, STK14A, Eukaryotic translation initiation factor 2 subunit alpha
Accession NO.: NP_004085.1
Protein Ino: 4758256
Official Symbol: EIF2S1
Geneid: 1965
Background: Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.
PubMed ID:http://aac.asm.org/content/39/7/1546.abstract
