Product Name: EGR4 Antibody
Species Reactivity: Dog, Human, Mouse, Rat
Tested Applications: ELISA, WB
Applications: EGR4 antibody can be used for detection of EGR4 by ELISA at 1:312500. EGR4 antibody can be used for detection of EGR4 by western blot at 0.0625 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 – 100,000.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight: 51 kDa
Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human EGR4.
Host Species: Rabbit
Purification: Antibody is purified by peptide affinity chromatography method.
Physical State: Lyophilized
CAS NO.: 173997-05-2
Product: Nepicastat
Buffer: Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.
Concentration: 1 mg/ml
Storage Conditions: For short periods of storage (days) store at 4˚C. For longer periods of storage, store EGR4 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: EGR4, NGFIC, NGFI-C, PAT133
Accession NO.: NP_001956
Protein Ino: 237512940
Official Symbol: EGR4
Geneid: 1961
Background: The nerve growth factor-induced clone C (NGFI-C/EGR4) gene is a zinc-finger transcription factor that is rapidly induced by nerve growth factor in rat pheochromocytoma PC12 cells and by seizure in brain. NGFI-C/EGR4 is closely related to the previously described early response genes, nerve growth factor-induced clone A (NGFI-A or EGR1), EGR2, and EGR3. These four early response (immediate early) proteins all contain very similar zinc-finger DNA binding domains and five highly homologous subdomains. EGR-4 functionally cooperate with NFAT proteins and induce expression of IL-2 and TNFalpha. Early growth response proteins (EGR) and nuclear factors of activated T cells (NFAT) form heterodimers and regulate proinflammatory cytokine gene expression.
PubMed ID:http://aac.asm.org/content/39/7/1472.abstract
