Product Name: EGF Receptor Antibody [B1D8]
Species Reactivity: Human
Tested Applications: Flow, Inhibition, IP
Applications: Functional Activity (Inhibition): antibody blocks EGF receptor activation inhibits A431 cell proliferation arrests tumor growth in vivo affinity purificationIP: 1-2µg/500ug protein lysateFlow Cytometry: 0.5-1 ug/million cellsThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.
User Note: Optimal dilutions for each application to be determined by the researcher
Predicted Molecular Weight:
Immunogen: Microsomes from A431 cells were used as the immunogen for this antibody.
Host Species: Mouse
Purification: Protein G purified monoclonal antibody
Physical State: Liquid
CAS NO.: 244218-51-7
Product: JTC-801
Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration: 0.2 mg/mL
Storage Conditions: Aliquot and Store at -20C. Avoid freez-thaw cycles.
Clonality: Monoclonal
Conjugate: Unconjugated
Alternate Names: Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, EGFR, ERBB, ERBB1, HER1
Accession NO.:
Protein Ino:
Official Symbol: EGF R
Geneid: 1956
Background: This antibody reacts with the extracellular domain of EGF receptor and blocks the EGF/TGFa induced activation. It also blocks tumor growth in vivo. It is excellent for purification of EGF receptor. EGFR is type I receptor tyrosine kinase with sequence homology to ErbB2, -3 -4 or HER2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGFa), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin.
PubMed ID:http://aac.asm.org/content/39/6/1272.abstract
