Product Name: EGF Receptor Antibody (biotin)
Species Reactivity: Human
Tested Applications: ELISA, WB
Applications: ELISA:Direct:To detect Human EGF Receptor by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25 – 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Human EGF Receptor.SandwichTo detect Human EGF Receptor by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 – 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci’s Polyclonal Anti-Human EGF Receptor (38-264) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Human EGF Receptor.Western Blot:To detect Human EGF Receptor by Western Blot analysis this antibody can be used at a concentration of 0.1 – 0.2 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human EGF Receptor is 1.5 – 3.0 ng/lane, under either reducing or non-reducing conditions.
User Note: Centrifuge vial prior to opening.
Predicted Molecular Weight:
Immunogen: Produced from sera of rabbits immunized with highly pure recombinant Human EGF Receptor. Human EGF Receptor specific antibody was purified by affinity chromatography and then biotinylated.
Host Species: Rabbit
Purification:
Physical State: Lyophilized
CAS NO.: 1009820-21-6
Product: CX-4945
Buffer:
Concentration:
Storage Conditions: The lyophilized antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-80˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.
Clonality: Polyclonal
Conjugate: Biotin
Alternate Names: ERBB, HER1, mENA, ERBB1, PIG61, ERBB, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1,
Accession NO.: P00533
Protein Ino: 2811086
Official Symbol: EGFR
Geneid: 1956
Background:
PubMed ID:http://aac.asm.org/content/39/6/1269.abstract
