Product Name: Dynamin (phospho Ser774) Antibody
Species Reactivity: Human, Mouse, Rat
Tested Applications: IF, IHC, WB
Applications: Dynamin antibody for phospho-Ser774 dynamin labels the purified protein phosphorylated in vitro by Cdk5 but not PKC (Tan, et al. 2003). The labeling of the dynamin band in Western blots of rat brain was blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. Sheep anti- Dynamin (Ser774) does not cross react with other purified substrates of CDK5 (e.g. amphiphysin and synapsin). Applications include Immunoblotting (IB), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blots (WB). Human, mouse and rat have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for IB and WB.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight: 95
Immunogen: Dynamin (Ser774) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho-Ser774 of human, mouse and rat dynamin.
Host Species: Sheep
Purification: Affinity Purified
Physical State: Liquid
CAS NO.: 857402-63-2
Product: Retaspimycin (Hydrochloride)
Buffer: 100 uL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mL BSA and 50% glycerol.
Concentration:
Storage Conditions: For long term storage -80˚C is recommended, but shorter term storage at -20˚C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stock solutions are stable for a minimum of 1 year at -20˚C.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: Dnm, Dnm, B-dynamin,
Accession NO.: P21575
Protein Ino: 190358918
Official Symbol: Dnm1
Geneid: 140694
Background: Dynamin is a member of a group of nerve terminal proteins called dephosphins that regulate synaptic vesicle endocytosis. Cyclin dependent protein kinase 5 phosphorylates dynamin at Ser774 and Ser778 that are the phosphorylation sites on dynamin phosphorylated in vivo. Phosphorylation of these sites on dynamin is thought to play a key role in synaptic vesicle trafficking.
PubMed ID:http://aac.asm.org/content/39/2/427.abstract
