Product Name: Duffy Antibody
Species Reactivity: Human
Tested Applications: ELISA, Flow, IHC-P, WB
Applications: ELISA: antibody detection limit dilution 1:16000. Western Blot: Approx 35kDa band observed in human kidney and human liver lysates (calculated MW of 35.6kDa according to NP_002027.2). Recommended concentration: 0.1-0.3μg/ml. Immunohistochemistry: In paraffin embedded human kidney stains epithelial cells of renal tubules and endothelial cells of vessels. The granular staining is compatible with the endoplasmic reticulum association of the protein. This antibody has also been Flow cytometry: A customer has reported successful staining of HEK293S-TetR transfected with DARC receptor at a dilution of 1/1000.
User Note:
Predicted Molecular Weight:
Immunogen: Duffy antibody was raised against a 19 amino acid synthetic peptide near the N-Terminus of Duffy.
Host Species: Goat
Purification: Duffy antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Physical State: Liquid
CAS NO.: 888216-25-9
Product: Ganetespib
Buffer: Duffy antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
Concentration: 500 ug/mL
Storage Conditions: Aliquot and store at -20˚C. Minimize freezing and thawing.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: FY, Duffy blood group, HGNC:4035, CCBP1, DARC, GPD, CTA-134P22.3, CD234, FY, OTTHUMP00000060081, chemokine receptor, Duffy blood group antigen, Glycoprotein D, ACKR1
Accession NO.: NP_002027.2
Protein Ino: 42822887
Official Symbol: DARC
Geneid: 2532
Background:
PubMed ID:http://aac.asm.org/content/39/1/221.abstract
