Product Name: Double Stranded DNA Antibody [SPM603]
Species Reactivity: Human
Tested Applications: Flow, ICC, IF, IHC-P
Applications: Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 1-2 ug/mlImmunocytochemistry (Acetone-fixed): 0.5-1 ug/ml for 30 min at RTImmunohistochemistry (FFPE): 1-2 ug/ml for 30 min at RT (1)Optimal dilution of the Double Stranded DNA antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
User Note: Optimal dilutions for each application to be determined by the researcher
Predicted Molecular Weight:
Immunogen: Nuclei of Burkitts cells were used as the immunogen for the Double Stranded DNA antibody.
Host Species: Mouse
Purification: Protein G affinity chromatography
Physical State: Liquid
CAS NO.: 1151830
Product: Fenspiride (Hydrochloride)
Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration: 0.2 mg/mL
Storage Conditions: Aliquot and Store at -20C. Avoid freez-thaw cycles.
Clonality: Monoclonal
Conjugate: Unconjugated
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Accession NO.:
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Geneid:
Background: Marker antibodies are useful in identification of organelles in cells, tissues, and biochemical preparations. This mAb recognizes the double stranded DNA in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in human cells. Double stranded (ds) DNA markers are useful tools in biology research and aid in the study of DNA behavior and characteristics.
PubMed ID:http://aac.asm.org/content/38/11/2655.abstract
