Product Name: cTnI Antibody [3A10A12]
Species Reactivity: Human
Tested Applications: ELISA, IHC, WB
Applications: Western Blot:Dilution 1:500 – 1:2,000IHC(P):Dilution 1:500 – 2,000IHC(F):Dilution 1:500 – 2,000ELISA:Propose dilution 1:10,000.Determining optimal working dilutions by titration test.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight:
Immunogen: Ni-NTA purified truncated recombinant cTnI expressed in E. Coli strain BL21 (DE3).
Host Species: Mouse
Purification:
Physical State:
CAS NO.: 525-79-1
Product: Kinetin
Buffer: Ascitic fluid containing 0.03% sodium azide.
Concentration:
Storage Conditions: cTnI monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Clonality: Monoclonal
Conjugate: Unconjugated
Alternate Names: TNNC1, Cardiac troponin ICMH7, RCM1, cTnI, CMD2A, TNNC1, CMD1FF
Accession NO.: P19429
Protein Ino: 136213
Official Symbol: TNNI3
Geneid: 7137
Background: cTnI has an apparent molecular weight of 22.5 kDa. cTnI is a candidate marker with acceptable sensitivity and specificity for AMI and other cardiac diseases. Troponin, a molecule that binds to the thin filament (actin) of striated muscle fibers, acts with intracellular calcium to control the interaction of the thin filament with the thick filament (myosin), thus regulating muscle contraction. Troponin I prevents muscle contraction in the absence of calcium, which has two skeletal muscle isoforms with considerable amino acid sequence homology. cTnI contains an additional N-terminal sequence and is highly specific for myocardium.
PubMed ID:http://aac.asm.org/content/37/6/1370.abstract
