Product Name: Clusterin Antibody (biotin)
Species Reactivity: Human
Tested Applications: ELISA, WB
Applications: ELISA: (direct and indirect: 1:2,000-1:5,000). Western Blot: (1:2,000-1:5,000 using ECL; suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk; suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight:
Immunogen: Recombinant human clusterin (nuclear form).
Host Species: Rabbit
Purification:
Physical State: Liquid
CAS NO.: 81403-80-7
Product: Alfuzosin
Buffer: Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.
Concentration: 1 mg/ml
Storage Conditions: Stable for at least 6 months after receipt when stored at -20˚C.
Clonality: Polyclonal
Conjugate: Biotin
Alternate Names: TRPM-2; Apolipoprotein J; APO-J; CLI; CLU; SGP-2
Accession NO.: NP_001822
Protein Ino: 42716297
Official Symbol: CLU
Geneid: 1191
Background: Clusterin shares homology with the small heat shock protein family of molecular chaperones. The mature secreted form of the protein is a glycosylated, 80-kDa disulfide-linked heterodimer of alpha and beta subunits (produced by internal cleavage). Clusterin is expressed in virtually all tissues and found in all human fluids. It is involved in numerous physiological processes important for carcinogenesis and tumor growth, including apoptotic cell death, cell cycle regulation, DNA repair, cell adhesion, tissue remodeling, lipid transportation, membrane recycling, and immune system regulation. Clusterin also exists as a nuclear protein. The secreted form of Clusterin has extracellular chaperone and anti-apoptotic activities while the nuclear form acts as a proapoptotic factor.
PubMed ID:http://aac.asm.org/content/36/8/1658.abstract
