Product Name: Claudin 1 Antibody
Species Reactivity: Dog, Human, Monkey, Mouse, Rat
Tested Applications: ELISA, IHC, WB
Applications: Claudin 1 antibody can be used in immunohistochemistry starting at 10 μg/mL.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight:
Immunogen: Claudin 1 antibody was raised against a synthetic peptide derived from the C-Terminus of Claudin 1 (Human).
Host Species: Rabbit
Purification: Immunoaffinity Chromatography
Physical State: Liquid
CAS NO.: 1191385-19-9
Product: B-Raf inhibitor 1 (dihydrochloride)
Buffer: 10 mM PBS, pH 7.6, 0.2% BSA, 15 mM sodium azide, before the addition of glycerol to 40%
Concentration:
Storage Conditions: Store Claudin 1 antibody at 4 ˚C or -20 ˚C. As with all antibodies avoid freeze/thaw cycles.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: CLDN1, Claudin-1, Claudin 1, ILVASC, SEMP1, CLD1
Accession NO.: O95832
Protein Ino: 6685283
Official Symbol: CLDN1
Geneid: 9076
Background: Claudin 1-8 proteins are a family of transmembrane proteins associated with tight junctions. Tight junctions are specialized regions of cell to cell contact; made up of network of strands to act as a molecular gasket for preventing the leakage of ions, water etc. between cells. They are abundant in luminal epithelial sheets where they maintain epithelial cell polarity. Different tissues exhibit different Claudin composition. Western Blot (Ab 2-4 μg/mL for 2 hrs at RT) Immunohistology (Formalin/paraffin) (1:100-1:200 for 30 min) Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min. Positive Control T47D cells, Skin or Breast carcinoma. Cellular Localization Cell membrane on skin and cytoplasmic & nuclear on breast carcinoma. Recognizes Claudin 1. Species Crossreactivity: Human, Monkey, Mouse, Canine, and Rat.
PubMed ID:http://aac.asm.org/content/36/4/902.abstract
