Product Name: Caspase-2 Antibody [11B4]
Species Reactivity: Dog, Human, Monkey, Mouse, Rat
Tested Applications: ELISA, Flow, ICC, IHC, IP, WB
Applications: Immunoprecipitation: (1-5ug/ml) . Western Blot: (1-2ug/ml) . Optimal conditions must be determined individually for each application.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight:
Immunogen: Recombinant human caspase-2 (p19 subunit).
Host Species: Rat
Purification: >95% (SDS-PAGE)
Physical State: Liquid
CAS NO.: 3818-50-6
Product: Bephenium (hydroxynaphthoate)
Buffer: Liquid. In PBS containing 0.02% sodium azide.
Concentration: 1 mg/ml
Storage Conditions: Stable for at least 1 year after receipt when stored at -20˚C.
Clonality: Monoclonal
Conjugate: Unconjugated
Alternate Names: CASP-2; Protease ICH-1; Neural Precursor Cell Expressed Developmentally Down-regulated Protein 2; NEDD-2
Accession NO.: P42575
Protein Ino: 83300977
Official Symbol: CASP2
Geneid: 835
Background: Caspase-2 is a Class I caspase with a long prodomain necessary for nuclear localization. Upon activation of the apoptotic pathway, the procaspase is cleaved into smaller fragments. Caspase-2 is the nuclear apoptotic respondent to cellular genotoxic stress or mitotic catastrophe and is involved in the activation cascade of caspases responsible for apoptosis execution. Activation occurs upon recruitment to a complex containing a p53-induced death domain protein, PIDD. This suggests caspase-2 can be a nuclear initiator caspase with a requirement for caspase-9 and caspase-3 activation in downstream apoptotic events. In apoptotic pathways resulting from UV-induced DNA damage, processing of caspase-2 occurs downstream of mitochondrial dysfunction and of caspase-9 and caspase-3 activation, extending a possible role for caspase-2 as a parallel effector caspase.
PubMed ID:http://aac.asm.org/content/43/5/1062.abstract
