Product Name: Caspase-2 Antibody [10C6]
Species Reactivity: Dog, Human, Monkey, Mouse, Rat
Tested Applications: ELISA, Flow, ICC, IHC
Applications: Flow Cytometry: (overexpressed in cell lines) . Immunocytochemistry: (10-20ug/ml; fix in 4% paraformaldehyde, 0.18% Triton-X100 for 10 min. prior to staining) . Immunohistochemistry: (frozen sections). Optimal conditions must be determined individually for each application.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight:
Immunogen: Recombinant human caspase-2 (p19 subunit).
Host Species: Rat
Purification: >95% (SDS-PAGE)
Physical State: Liquid
CAS NO.: 301326-22-7
Product: CH-223191
Buffer: Liquid. In PBS containing 0.02% sodium azide.
Concentration: 1 mg/ml
Storage Conditions: Stable for at least 1 year after receipt when stored at -20˚C.
Clonality: Monoclonal
Conjugate: Unconjugated
Alternate Names: CASP-2; Protease ICH-1; Neural Precursor Cell Expressed Developmentally Down-regulated Protein 2; NEDD-2
Accession NO.: P42575
Protein Ino: 83300977
Official Symbol: CASP2
Geneid: 835
Background: Caspase-2 is a Class I caspase with a long prodomain necessary for nuclear localization. Upon activation of the apoptotic pathway, the procaspase is cleaved into smaller fragments. Caspase-2 is the nuclear apoptotic respondent to cellular genotoxic stress or mitotic catastrophe and is involved in the activation cascade of caspases responsible for apoptosis execution. Activation occurs upon recruitment to a complex containing a p53-induced death domain protein, PIDD. This suggests caspase-2 can be a nuclear initiator caspase with a requirement for caspase-9 and caspase-3 activation in downstream apoptotic events. In apoptotic pathways resulting from UV-induced DNA damage, processing of caspase-2 occurs downstream of mitochondrial dysfunction and of caspase-9 and caspase-3 activation, extending a possible role for caspase-2 as a parallel effector caspase.
PubMed ID:http://aac.asm.org/content/43/5/1314.abstract
