Product Name: Caspase-12 Antibody [12G6]
Species Reactivity: Mouse
Tested Applications: ELISA, Flow, WB
Applications: ELISA: (direct and indirect: 1:2,000-1:5,000). Immunoprecipitation: (1:500-1:5,000). Western Blot: (1:500-1:5,000 using ECL. Suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight:
Immunogen: Recombinant mouse caspase-12 (p20 fragment) and synthetic peptide corresponding to aa 183-205 of mouse caspase-12.
Host Species: Rat
Purification: >95% (SDS-PAGE)
Physical State: Liquid
CAS NO.: 738606-46-7
Product: ETC-1002
Buffer: Liquid. In PBS containing 0.02% sodium azide.
Concentration: 1 mg/ml
Storage Conditions: Stable for at least 1 year after receipt when stored at -20˚C.
Clonality: Monoclonal
Conjugate: Unconjugated
Alternate Names: CASP-12
Accession NO.: CAA73532
Protein Ino: 2094806
Official Symbol: Casp12
Geneid: 12364
Background: Caspase-12 is a member of the ICE/CED-3 family of caspases and is detected as a protein of ~51kDa. Overexpression of caspase-12 in cells induces apoptosis. Capase-12 is localized to the endoplasmatic reticulum (ER) and activated by ER stress and specifically cleaves and activates pro-caspase-9 and is also thought to be involved in the apoptosis induced by viral infection. Caspase-12-deficient mice are resistant to ER stress-induced apoptosis. Caspase-12 is probably expressed in many and perhaps all mouse tissues and is expressed at high levels in skeletal muscle, heart, lung and kidney and at moderate levels in brain and liver. In many humans caspase-12 is a pseudo gene and not expressed.
PubMed ID:http://aac.asm.org/content/43/5/1124.abstract
