Product Name: Cardif Antibody [Adri-1]
Species Reactivity: Human
Tested Applications: ICC, IHC, IP, WB
Applications: Immunocytochemistry: (1:100). Immunohistochemistry: (paraffin sections). Immunoprecipitation: (1:200). Western Blot: (1:1,000).
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight:
Immunogen: Recombinant human Cardif (aa 160-450).
Host Species: Mouse
Purification: >95% (SDS-PAGE)
Physical State: Liquid
CAS NO.: 632-85-9
Product: Wogonin
Buffer: Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.
Concentration: 1 mg/ml
Storage Conditions: Stable for at least 1 year after receipt when stored at -20˚C.
Clonality: Monoclonal
Conjugate: Unconjugated
Alternate Names: CARD Adapter Inducing Interferon-beta; IPS-1; MAVS; Mitochondrial Antiviral Signaling Protein; VISA; Virus-induced Signaling Adapter; Interferon-beta Promoter Stimulator Protein 1
Accession NO.: NP_065797
Protein Ino: 83776598
Official Symbol: MAVS
Geneid: 57506
Background: RIG-I (retinoic acid-inducible gene I; Ddx58) and MDA5 (melanoma differentiation-associated gene 5, also known as Ifih1 or Helicard) are proteins that sense viral replication intermediates, such as double-stranded RNA and triggers the host antiviral programs. These molecules signal the downstream activation of NF-kappaB and IFN regulatory factor (IRF) -3, which coordinately regulate the expression of type-I interferons. Cardif (also called VISA/IPS-1/MAVS) is a new CARD (caspase activation and recruitment domain)-containing adaptor protein that interacts with the CARD domain of RIG-I and MDA5, leading to the activation of NF-kappaB and IRF3. Cardif is located to the mitochondrial outer membrane. Removal of the mitochondrial-targeting domain of cardif abolishes its ability to induce IFNs. Cardif is cleaved and inactivated by NS3-4A, a serine protease from hepatitis C virus known to block interferon-beta production.
PubMed ID:http://aac.asm.org/content/43/4/902.abstract
