Product Name: CARD11 Antibody
Species Reactivity: Human
Tested Applications: ELISA, IHC-P, WB
Applications: ELISA: antibody detection limit dilution 1:32000. Western Blot: Approx 150+65kDa bands observed in lysates of cell line Jurkat (calculated MW of 133kDa according to NP_115791.2). A similar pattern was observed in HEK293 transiently expressing CARD11 (pciture not shown). These bands are not observed in Immunohistochemistry: In paraffin embedded Human Spleen shows nuclear staining. Recommended concentration, 2-5μg/ml.
User Note:
Predicted Molecular Weight:
Immunogen: CARD11 antibody was raised against a 12 amino acid synthetic peptide near the C-Terminus of CARD11.
Host Species: Goat
Purification: CARD11 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Physical State: Liquid
CAS NO.: 1373423-53-0
Product: GSK-J4
Buffer: CARD11 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
Concentration: 500 ug/mL
Storage Conditions: Aliquot and store at -20˚C. Minimize freezing and thawing.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: CARD11, caspase recruitment domain family, member 11, BIMP3, CARMA1, MGC133069, card-maguk protein 1, bcl10-interacting maguk protein 3
Accession NO.: NP_115791.2
Protein Ino: 16507952
Official Symbol: CARD11
Geneid: 84433
Background:
PubMed ID:http://aac.asm.org/content/43/4/769.abstract