Product Name: Calreticulin Antibody [CR213-2AG]
Species Reactivity: Human
Tested Applications: ELISA, WB
Applications: ELISA: (direct and indirect: 1:2,000-1:5,000). Western Blot: (1:2,000-1:5,000 using ECL. Suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight:
Immunogen: Recombinant human calreticulin.
Host Species: Mouse
Purification:
Physical State: Liquid
CAS NO.: 1531634-31-7
Product: AF38469
Buffer: Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.
Concentration: 1 mg/ml
Storage Conditions: Stable for at least 1 year after receipt when stored at -20˚C.
Clonality: Monoclonal
Conjugate: Unconjugated
Alternate Names: CRP55; Calregulin; Endoplasmic Reticulum Resident Protein 60; ERp60; grp60
Accession NO.: NP_004334
Protein Ino: 4757900
Official Symbol: CALR
Geneid: 811
Background: Calreticulin is involved in regulation of intracellular Ca2+ homoeostasis and ER Ca2+ capacity. It constitutes together with calnexin and ERp57 the ‘calreticulin/calnexin cycle’ that is responsible for folding and quality control of newly synthesized glycoproteins. Calreticulin has been implicated to play a role in many biological systems, including functions inside and outside the ER, indicating that the protein is a multi-process molecule.
PubMed ID:http://aac.asm.org/content/43/2/424.abstract
