Product Name: Anthrax Lethal Factor Antibody
Species Reactivity: Bacteria
Tested Applications: ELISA
Applications: Anthrax lethal factor antibody can be used for the detection of Anthrax LF protein in ELISA. It will detect 10 ng of free peptide at 1 μg/mL.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight:
Immunogen: Anthrax lethal factor antibody was raised against a synthetic peptide corresponding to 16 amino acids in the middle of the Anthrax lethal factor protein.The immunogen is located within amino acids 420 – 470 of Anthrax Lethal Factor.
Host Species: Rabbit
Purification: Anthrax Lethal Factor Antibody is affinity chromatography purified via peptide column.
Physical State: Liquid
CAS NO.: 1313881-70-7
Product: ARQ-092
Buffer: Anthrax Lethal Factor Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Conditions: Anthrax Lethal Factor antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: Anthrax Lethal Factor Antibody: Lethal factor, Anthrax lethal toxin endopeptidase component, LF
Accession NO.: P15917
Protein Ino: 50402185
Official Symbol: pxo1_107
Geneid: 3361711
Background: Anthrax Lethal Factor Antibody: Anthrax infection is initiated by the inhalation, ingestion, or cutaneous contact with Bacillus anthracis endospores. B. anthracis produces three polypeptides that comprise the anthrax toxin: protective antigen (PA), lethal factor (LF), and edema factor (EF). PA binds to two related proteins on the cell surface; these are termed tumor epithelial marker 8 (TEM8)/anthrax toxin receptor (ATR) and capillary morphogenesis protein 2 (CMG2), although it is still unclear which is physiologically relevant. Following PA binding to its receptor, PA is cleaved into two fragments by a furin-like protease. The bound fragment binds both LF and EF; the resulting complex is then endocytosed which allows the translocation of LF and EF into the cytoplasm. LF is the primary toxin of anthrax and functions as a highly specific protease that cleaves members of the mitogen-activated protein kinase kinase (MAPKK) family near their amino terminus, interfering with MAPK signaling and inducing apoptosis.
PubMed ID:http://aac.asm.org/content/53/1/136.abstract
