Product Name: eIF4G (Ab 1232) Antibody
Species Reactivity: Human, Mouse, Rat
Tested Applications: IF, IHC, WB
Applications: Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
User Note:
Predicted Molecular Weight: 220 kDa
Immunogen: eIF4G (Ab-1232) antibody was raised against a peptide sequence around aa.1230~1234 (P-V-S-P-L) derived from Human eIF4G.
Host Species: Rabbit
Purification: Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Physical State: Liquid
CAS NO.: 107233-08-9
Product: Cevimeline
Buffer: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration: 1 mg/mL
Storage Conditions: Store antibody at -20˚C for up to one year.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: P220, EIF4F, EIF4G, EIF4GI, PARK18, EIF-4G1, GAPD, CDABP0047, OK/SW-cl.12,
Accession NO.: NP_004944.2
Protein Ino: 38201619
Official Symbol: EIF4G1
Geneid: 1981
Background: eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. Interacts with eIF3, mutually exclusive with EIF4A1 or EIFA2, EIF4E and through its N-terminus with PAPBC1. Interacts through its C-terminus with the serine/threonine kinases MKNK1, and with MKNK2. Appears to act as a scaffold protein, holding these enzymes in place to phosphorylate EIF4E. Non-phosphorylated EIF4EBP1 competes with EIF4G1/EIF4G3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. EIF4G1/EIF4G3 interacts with PABPC1 to bring about circularization of the mRNA. Rapamycin can attenuate insulin stimulation mediated by FKBPs. Interacts with EIF4E3. Interacts with MIF4GD. Interacts with rotavirus A NSP3; in this interaction, NSP3 takes the place of PABPC1 thereby inducing shutoff of host protein synthesis
PubMed ID:http://aac.asm.org/content/39/8/1772.abstract
