Product Name: EpCAM Antibody [EGP40/1798]
Species Reactivity: Human
Tested Applications: ELISA, Flow, IF, IHC-P, WB
Applications: ELISA: 2-4 ug/ml; order the BSA free formulation for coatingWB: 0.5-1 ug/mlIF: 1-2 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RTFlow Cytometry: 0.5-1 ug/million cells in 0.1mlTitering of the Ep-CAM antibody may be required for optimal performance.
User Note: Optimal dilutions for each application to be determined by the researcher
Predicted Molecular Weight:
Immunogen: A human recombinant partial protein corresponding to amino acids 77-202 (extracellular domain) was used as the immunogen for the Ep-CAM antibody.
Host Species: Mouse
Purification: Protein G affinity chromatography
Physical State: Liquid
CAS NO.: 1311385-32-6
Product: LY2979165
Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration: 0.2 mg/mL
Storage Conditions: Aliquot and Store at -20C. Avoid freez-thaw cycles.
Clonality: Monoclonal
Conjugate: Unconjugated
Alternate Names: Epithelial cell adhesion molecule, Ep-CAM, Adenocarcinoma-associated antigen, Cell surface glycoprotein Trop-1, Epithelial cell surface antigen, Epithelial glycoprotein, EGP, Epithelial glycoprotein 314, EGP314, hEGP314, KS 1/4 antigen, KSA, Major gastrointestinal tumor-associated protein GA733-2, Tumor-associated calcium signal transducer 1, CD326, EPCAM, GA733-2, M1S2, M4S1, MIC18, TACSTD1, TROP1
Accession NO.:
Protein Ino:
Official Symbol: EPCAM
Geneid: 4072
Background: EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.
PubMed ID:http://aac.asm.org/content/39/12/2678.abstract
