Product Name: Eotaxin-2 Antibody
Species Reactivity: Mouse
Tested Applications: ELISA, Neut, WB
Applications: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of mEotaxin-2 (100 ng/mL), a concentration of 7.0-8.0 μg/mL of this antibody is required. ELISA:To detect murine Eotaxin-2 by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 – 0.4 ng/well of recombinant murine Eotaxin-2.Sandwich: To detect mEotaxin-2 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 – 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Murine Eotaxin-2 (XP-5125Bt) as a detection antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant mEotaxin-2. Western Blot:To detect murine Eotaxin-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 – 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant murine Eotaxin-2 is 1.5 – 3.0 ng/lane, under either reducing or non-reducing conditions.
User Note: Centrifuge vial prior to opening.
Predicted Molecular Weight:
Immunogen: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant murine Eotaxin-2.
Host Species: Goat
Purification: Anti-murine Eotaxin-2 specific antibody was purified by affinity chromatography employing immobilized murine Eotaxin-2 matrix.
Physical State: Lyophilized
CAS NO.: 220904-83-6
Product: GW 5074
Buffer:
Concentration:
Storage Conditions: Eotaxin-2 antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: CKb-6, MPIF-2, Scya24, C-C motif chemokine 24, Eosinophil chemotactic protein 2,
Accession NO.: Q9JKC0
Protein Ino: 13431902
Official Symbol: Ccl24
Geneid: 56221
Background: Eotaxin is a 74 amino acid, eosinophil chemotactic CC chemokine originally found in bronchoalveolar lavage fluid from allergic inflammatory subjects. It is involved in regulating the recruitment and activation of inflammatory leukocytes, particularly eosinophils. Eotaxin binds and activates the CCR3 chemokine receptor, and may play a fundamental role in the development of allergic responses.
PubMed ID:http://aac.asm.org/content/39/11/2541.abstract
