Product Name: EPS15 Antibody
Species Reactivity: Human
Tested Applications: WB
Applications: For WB starting dilution is: 1:1000
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight: 99 kDa
Immunogen: This EPS15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 840-870 amino acids from the C-terminal region of human EPS15.
Host Species: Rabbit
Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Physical State: Liquid
CAS NO.: 942947-93-5
Product: CCT129202
Buffer: Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration: 2 mg/ml
Storage Conditions: Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: Epidermal growth factor receptor substrate 15, Protein Eps15, Protein AF-1p, EPS15, AF1P
Accession NO.: P42566
Protein Ino: 67476728
Official Symbol: EPS15
Geneid: 2060
Background: EPS15 is involved in cell growth regulation, possibly via the regulation of mitogenic signals and control of cell proliferation. EPS15 also participates in the internalization of ligand-inducible receptors of the receptor tyrosine kinase (RTK) type, in particular EGFR. Potential interaction partners include AP2A2, STN2, EPN1, and CRK via its SH3-binding sites. EPS15 is ubiquitously expressed. Phosphorylation on Tyr-849 is involved in the internalization of EGFR. EPS15 is not required for membrane translocation after EGF treatment or for targeting to coated pits, but essential for a subsequent step in EGFR endocytosis. This protein is involved in a t(1;11)(p32;q23) chromosomal translocation in acute leukemias causing fusion to the trithorax (MLL or HRX) gene product which contains DNA-binding motifs resulting in a rogue activator protein. Structurally, EPS15 contains 2 EF-hand calcium-binding domains, 3 EH domains, and 2 ubiquitin-interacting motif (UIM) repeats.
PubMed ID:http://aac.asm.org/content/40/1/115.abstract
