Product Name: ELF3 Antibody
Species Reactivity:
Tested Applications:
Applications: ELF3 antibody can be used for detection of ELF3 by ELISA at 1:312500. ELF3 antibody can be used for detection of ELF3 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 – 100,000.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight: 41 kDa
Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ELF3.
Host Species: Rabbit
Purification: Antibody is purified by protein A chromatography method.
Physical State: Lyophilized
CAS NO.: 870070-55-6
Product: Tozadenant
Buffer: Antibody is lyophilized in PBS buffer with 2% sucrose. Add 100 μL of distilled water. Final antibody concentration is 1 mg/mL.
Concentration: 1 mg/ml
Storage Conditions: For short periods of storage (days) store at 4˚C. For longer periods of storage, store ELF3 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: ELF3, ERT, ESX, EPR-1, ESE-1
Accession NO.: NP_004424
Protein Ino: 167235023
Official Symbol: ELF3
Geneid: 1999
Background: ELF3 is a novel, highly tissue-restricted member of the ets transcription factor/oncogene family. ELF3 contains two putative DNA binding domains: an ETS domain, which is unique in that the 5 half shows relatively weak homology to known ets factors, and an A/T hook domain, found in HMG proteins and various other nuclear factors. In contrast to any known ets factors, ELF3 is expressed exclusively in epithelial cells. ELF3 is expected to be a critical regulator of epithelial cell differentiation. ETS factor ELF3 is a transcriptional regulator of angiopoietin-1 gene regulation in the setting of inflammation. ELF3 overexpressed early during human breast tumorigenesis. ELF3 expression confers a transformed and in vitro metastatic phenotype to otherwise normal MCF-12A cells. ELF3 mediates the expression of TGF-beta RII, and the transcriptional inhibition of ets-related transcription factor could be a one of the mechanisms of colonic carcinogenesis. ELF3 is positively and negatively modulated by other interacting proteins including Ku70, Ku86, p300, and CBP.
PubMed ID:http://aac.asm.org/content/39/8/1881.abstract
