Product Name: EIF4G2 Antibody [39C534.1]
Species Reactivity: Human, Mouse
Tested Applications: IHC, WB
Applications: EImmunofluorescence4G2 antibody can be used in ELISA, Western Blot, immunohistochemistry starting at 5 μg/mL, and immunofluorescence starting at 10 μg/mL.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight:
Immunogen: EIF4G2 monoclonal antibody was raised against amino acids 796 – 812 of EIF4G2 (Human).
Host Species: Mouse
Purification: Protein G Column
Physical State: Liquid
CAS NO.: 21343-40-8
Product: Ercalcidiol
Buffer: PBS, 0.2% gelatin, 0.05% sodium azide.
Concentration:
Storage Conditions: EIF4G2 antibody can be stored short term 4 ˚C. For long term storage aliquot and store at -20 ˚C. As with all antibodies avoid freeze/thaw cycles.
Clonality: Monoclonal
Conjugate: Unconjugated
Alternate Names: EIF4G2, AAG1, Aging-associated protein 1, DAP-5, DAP5, Death-associated protein 5, EIF-4G 2, EIF-4-gamma 2, EIF4G 2, Translation repressor nat1, p97
Accession NO.: P78344
Protein Ino: 30315906
Official Symbol: EIF4G2
Geneid: 1982
Background: Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Recently, five novel apoptotic genes (named DAP genes for: Death Associated Proteins) have been identified that code for proteins, which display a diverse spectrum of biochemical activities. These include a novel type of calcium/calmodulin-regulated kinase, which carries ankyrin repeats and a death domain (DAP-kinase), a nucleotide-binding protein (DAP-3), a small proline-rich cytoplasmic protein (DAP-1), and a novel homolog of the eIF4G translation initiation factor (DAP-5) (1-2). DAP-5 (also named p97 and NAT1) is a ubiquitously expressed member of the translation initiation factor eIF4G family that lacks the eIF4E binding site. DAP-5 was cloned from HeLa cells using a functional approach in attempt to isolate cDNA fragments, which convey resistance to gamma interferon (IFN-g)-induced programmed cell death. A fragment of the cDNA when expressed as a mini-protein of 28 kDa protects the cell from IFN-g induced apoptosis. The full-length DAP-5 cDNA codes for a protein of approximate molecular weight of 97 kDa. Low level of expression protects the cells, whereas high level of expression results in cell death (3-4).
PubMed ID:http://aac.asm.org/content/39/8/1790.abstract
